ABSTRACT
ABSTRACT Liquid perfluorocarbon (PFC) instillation has been studied experimentally as an adjuvant therapy in the preservation of lung grafts during cold ischemia. The objective of this study was to evaluate whether vaporized PFC is also protective of lung grafts at different cold ischemia times. We performed histological analysis of and measured oxidative stress in the lungs of animals that received only preservation solution with low-potassium dextran (LPD) or vaporized PFC together with LPD. We conclude that vaporized PFC reduces the production of free radicals and the number of pulmonary structural changes resulting from cold ischemia.
RESUMO O perfluorocarbono (PFC) líquido tem sido estudado experimentalmente como uma substância adjuvante na preservação de enxertos pulmonares durante o período de isquemia fria. O objetivo deste estudo foi avaliar se o PFC vaporizado (e não instilado) também atuaria como protetor de enxertos pulmonares em diferentes tempos de isquemia fria. Realizamos análise histológica e dosamos o estresse oxidativo em pulmões de animais que receberam somente uma solução de preservação com low-potassium dextran (LPD, dextrana com baixa concentração de potássio) ou PFC vaporizado associado a LPD. Concluímos que o PFC vaporizado reduziu a produção de radicais livres e provocou menor número de alterações estruturais pulmonares decorrentes do período de isquemia fria que o uso de LPD isoladamente.
Subject(s)
Humans , Organ Preservation/methods , Lung Transplantation/methods , Oxidative Stress/drug effects , Cold Ischemia/methods , Fluorocarbons/pharmacology , Lung/drug effects , Reference Values , Time Factors , Reproducibility of Results , Dextrans/pharmacology , Organ Preservation Solutions , Glucose/pharmacology , Lung/pathologyABSTRACT
Abstract Purpose: To evaluate the concentration of transforming growth factor beta 1 (TGFB1) levels in a rat pleural effusion obtained by inoculation of intrapleural bacteria or turpentine through thoracentesis. Methods: Thirty-Nine Wistar rats were divided into three groups: Staphylococcus aureus (SA, n = 17); Streptococcus pneumoniae (SP, n = 12); and turpentine (control, n = 10). Pleural fluid was collected through ultrasound-guided thoracentesis 12 h, 24 h, and 36 h after instillation of bacteria or turpentine. Levels of TGFB1 were measured in pleural fluid. Results: At 12 h, mean TGFB1concentrations were 5.3450 pg/mL in the SA group, 5.3449 pg/mL in the SP group, and 5.3450 pg/mL in controls. At 24 h, they were 4.6700 pg/mL in the SA group, 4.6700 pg/mL in the SP group, and 4.6700 pg/mL in controls. At 36 h, they were 4.6699 pg/mL in the SA group and in control. No difference was observed among the groups in mean TGFB1concentration (p = 0.12); however, a significant intragroup reduction in mean TGFB1 was observed between 12 and 24 h (p < 0.01). Conclusion: The transforming growth factor beta 1 concentrations were not useful as a diagnostic tool or an early marker of infected pleural effusion.
Subject(s)
Animals , Male , Rats , Pleural Effusion/diagnosis , Empyema, Pleural/diagnosis , Transforming Growth Factor beta1/analysis , Pleural Effusion/complications , Bacteria/pathogenicity , Biomarkers/analysis , Empyema, Pleural/complications , Empyema, Pleural/microbiology , Rats, Wistar , Disease Models, AnimalABSTRACT
A infecçäo por Capilária hepática é rara em humanos sendo, a maioria dos casos, diagnosticados à autópsia. Os autores relatam um caso de diagnóstico histopatológico em um menino de 17 meses; apresentam sua evoluçäo clínico-laboratorial e destacam o sucesso obtido com a terapêutica empregada
Subject(s)
Infant , Humans , Male , Nematode Infections/pathology , Capillaria , Follow-Up Studies , Nematode Infections/drug therapy , Nitrophenols/therapeutic use , Prednisone/therapeutic use , Pyrantel Tartrate/therapeutic useABSTRACT
Discutem-se os achados da autópsia de uma criança de dois anos de idade, feminina, portadora de retinoblastoma unilateral, com enucleaçäo prévia, que apresentou propagaçäo para a regiäo supra-selar, além de metástases à distância
Subject(s)
Child, Preschool , Humans , Female , Cranial Nerve Neoplasms/secondary , Eye Neoplasms/pathology , Optic Nerve Diseases/pathology , Retinoblastoma/pathology , Cranial Nerve Neoplasms/pathology , Subarachnoid SpaceABSTRACT
O neuroblastoma é um dos tumores sólidos mais comuns no grupo pediátrico, contribuindo com aproximadamente 50% das neoplasias neonatais. A supra-renal é o sítio primário mais freqüentemente acometido. No presente trabalho, os autores realizam uma revisäo bibliográfica sobre neuroblastomas da supra-renal, dando ênfase ao diagnóstico, tratamento e prognóstico